Anti-flavin antibodies

Anti-flavin antibodies.

Antibodies were elicited to FAD by using the hapten N-6-(6-aminohexyl)-FAD conjugated to the immunogenic carrier protein bovine serum albumin. Cross-reactivity was determined by Ouchterlony double-diffusion analysis with N-6-(6-aminohexyl)-FAD coupled to rabbit serum albumin. Anti-FAD IgG was partially purified by (NH4)2SO4 precipitation followed by DEAE-cellulose/CM-cellulose and bovine serum ...

Phage antibodies against an unstable hapten: oxygen sensitive reduced flavin.

It is difficult to raise antibodies against haptens and antigens that are unstable under the physiological conditions of the serum. Here we have used a phage antibody library to isolate antibody fragments against oxygen sensitive reduced flavin, by selection of the phage under anaerobic and reducing conditions at pH 5 and a pre-elution step with the oxidized flavin. The binding of the reduced h...

Anti - Phosphocholine Hybridoma Antibodies

Three major groups of antibodies have been identified during the analysis of the clonal response to phosphoeholine (PC). t The predominant one shows multiple molecular features of the PC-binding myeloma protein (PC-BMP) TEPC 15 (1-3), and two others possess idiotypes (Id) and L-chain characteristics of two other PCBMP, M511 (2, 4) and M603 (5). All three Id groups are conserved in inbred mice (...

Detection of Anti-Ganglioside Antibodies in Guillain-Barre Syndrome

Anti-phospholipid/Cofactor Antibodies

Following a meeting at Sapporo, Japan, an international consensus statement on preliminary classification criteria for definite anti-phospholipid syndrome was published.1 This set of criteria is divided into the clinical part which includes vascular thrombosis and pregnancy morbidity and the laboratory part which includes anti-cardiolipin (aCL) antibody and lupus anti-coagulant tests. Diagnosis...